Flow cytometric analysis of T lymphocytes and cytokines in aqueous humor of patients with varicella zoster virus-mediated acute retinal necrosis
نویسندگان
چکیده
Abstract Background The purpose of this study is to investigate the aqueous humor (AH) T lymphocyte subsets and cytokines acute retinal necrosis (ARN) elucidate immunologic inflammatory features disorder. Methods Three patients with ARN infected varicella zoster virus (VZV) who underwent multiple intravitreal injections ganciclovir were enrolled in study. control group consisted four non-infectious anterior uveitis (AAU). Flow cytometric analysis was performed on from AH peripheral blood (PB) samples during active phase intraocular inflammation. Five measured each sample various clinical characteristics also assessed. Results VZV deoxyribonucleic acid (DNA) detected by real-time polymerase chain reaction (PCR) all patients, showed higher CD8+ lymphocytes population than AAU ( p = 0.006). CD4/CD8 ratios percentage CD8 + CD25+ significantly lower 0.006; 0.012). In percentages CD4+ those found PB. CD4 proportion PB 0.001). Immunoregulatory cytokine Interleukin-10 elevated comparison case 0.036). ARN, copy number DNA positively correlated negatively ratio course disease treatment 0.009, r 0.92; 0.039, ? 0.834). Conclusion caused had different profile patients. High low cell may be a potential biomarker for diagnosis viral-infectious uveitis. examination at sites has become useful research tool differentiating viral non-viral
منابع مشابه
Genetic analysis of varicella-zoster virus in the aqueous humor in uveitis with severe hyphema
BACKGROUND Genetic variations have been identified in the genome of varicella-zoster virus (VZV) strains using vesicle fluid, varicella scabs and throat swab samples. We report a rare case of VZV-associated uveitis with severe hyphema, which was immediately diagnosed by polymerase chain reaction (PCR) using the aqueous humor, in which we were able to analyze the VZV genotype for the first time....
متن کاملVariable R1 region in varicella zoster virus in fulminant type of acute retinal necrosis syndrome.
BACKGROUND/AIMS Varicella zoster virus (VZV) is a causative agent in acute retinal necrosis (ARN) syndrome. However, in spite of aggressive antiviral therapy, clinical characteristics among patients have varied. Different viral strains were examined to determine their respective role in producing clinical characteristics. The viral strains were also compared with those of previously reported on...
متن کاملDetection of varicella-zoster virus genome in the vitreous humor from two patients with acute retinal necrosis; lacking or having a PstI cleavage site.
Using polymerase chain reaction, we detected the varicella-zoster virus genome in the vitreous humor of two patients with clinically diagnosed acute retinal necrosis. One of the two cases was thought to be caused by infection with a varicella-zoster virus lacking a PstI cleavage site. We could not find any clinical differences between the two substrains. The presence of a PstI cleavage site on ...
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ژورنال
عنوان ژورنال: BMC Ophthalmology
سال: 2021
ISSN: ['1471-2415']
DOI: https://doi.org/10.1186/s12886-021-01951-1